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KMID : 1177720100040020005
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Journal of Alternatives to Animal Experiments
2010 Volume.4 No. 2 p.5 ~ p.13
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Interlaboratory Validation of KeraSkin(TM) for Replacement of Skin Irritation Test Using Animal Model
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Moon Ji-Young
Lee Su-Hyon
Jung Haeng-Sun
Ryu Yang-Hwan
Jung Kyoung-Mi
Lim Kyung-Min
Park Cheol-Beom
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Abstract
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Reconstructed skin provides advantages to single cell culture testing and leads to promising results as shown by different validation studies. In this study we investigated the interlaboratory validation for the efficacy of “KeraskinTM” model. We conducted endpoint analysis including cell viability (MTT reduction) and IL-1α release. Eighteen item, which were ECVAM test meterial, were selected for this validation study. Results of MTT assay were not different between EPI-200 and KeraSkinTM, but IL-1α levels of some test items different between EPI-200 and KeraSkinTM. We concluded that this KeraSkinTM model is useful for alternative skin irritation test, although other tests should be conducted for many other chemicals in international validation study.
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KEYWORD
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Skin Irritation, EPI-200, KeraSkin, Alternative Method
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